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| Albany Middle School | B | 8 | 8 | Alameda Regional Science Olympiad | Albany USD | CA |
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| BASIS Cedar Park | B | 1 | | Texas UT Austin | Charter | TX |
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| Creekside MS | B | 2 | 2 | Alameda Regional Science Olympiad | Castro Valley USD | CA | |
| Kraemer Middle School | B | 4 | 4 | Orange County Regional | Placentia Yorba Linda Unified | CA |
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| Lakeside Middle School | B | 1 | | SoCal Joint Regional | Irvine Unified School District | CA |
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| Pleasantville Middle School | B | 5 | | Lower Hudson Valley | Pleasantville Union Free School District | NY |
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| Questa | B | 1 | 1 | San Joaquin County | Lammersville USD | CA |
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| River Trail Middle School | B | 3 | 3 | Regional Placeholder | Fulton County | GA |
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| Springhouse Middle School | B | 1 | | Central East Region at Kutztown University | Parkland | PA |
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| Venado Middle School | B | 1 | | Southern California | Irvine Unified School District | CA |
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| Wilbur Wright Middle School | B | 1 | 1 | Purdue University Northwest Regional | School Town of Munster | IN |
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