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| Allen County-Scottsville High School | C | 2 | 2 | Somerset Regional | Allen County Schools |
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| Ballard High School | C | 1 | 1 | Louisville Regional | Jefferson County Public Schools | |
| Barbourville Independent High School | C | 1 | 1 | Somerset Regional | Barbourville Independent Schools | |
| Barbourville Middle School | B | 1 | 1 | Somerset Regional | Barbourville Independent | |
| Bell County High School | C | 2 | 2 | Somerset Regional | Bell County Schools | |
| Bowling Green High School | C | 1 | 1 | Kentucky Science Olympiad | Bowling Green Independent | |
| Christian Academy of Louisvile | C | 1 | 1 | Lexington Regional | Jefferson County | |
| Crosby Middle School | B | 1 | 1 | Kentucky Science Olympiad | Jefferson County Public Schools | |
| duPont Manual High School | C | 4 | | Louisville Regional | Jefferson County Public Schools |
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| Farristown Middle School | B | 1 | 1 | Kentucky Science Olympiad | Madison County School District | |
| Francis Parker School of Louisville | C | 1 | 1 | Louisville Regional | Independent | |
| Greenup County High School | C | 1 | | Maysville Regional | Greenup County | |
| Highlands Latin School | B | 1 | 1 | Louisville Regional | N/A |
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| Highlands Latin School | C | 1 | 1 | Louisville Regional | NA |
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| Hopkinsville High School | C | 1 | 1 | Louisville Regional | Christian County | |
| J. Graham Brown School | C | 1 | 1 | Louisville Regional | Jefferson County Public Schools | |
| James E Bazzell Middle School | B | 2 | 2 | Somerset Regional | Allen Co |
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| Kentucky Country Day School | C | 1 | 1 | Louisville Regional | N/A | |
| Knox Central High School | C | 2 | 2 | Invitational Only | Knox County Public Schools | |
| Larry A. Ryle High School | C | 1 | 1 | Louisville Regional | Boone County Schools | |
| Madison Central High School | C | 2 | | Invitational Only | Madison County | |
| Madisonville | C | 1 | 1 | Louisville Regional | Hopkins County School District | |
| Meyzeek Middle School | B | 3 | 3 | Louisville Regional | Jefferson county Public Schools |
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| Middlesboro High School1 | C | 1 | | Kentucky Science Olympiad | Middlesboro Independent | |
| middlesboro middle | B | 1 | 1 | Kentucky Science Olympiad | middlesboro middle | |
| Morgan County High School | C | 1 | 2 | Lexington Regional | Morgan County School District | |
| Noe Middle School | B | 2 | 2 | Louisville Regional | Jefferson County Schools | |
| North Oldham Middle School | B | 2 | 2 | Louisville Regional | Oldham County School District | |
| Owen County High School | C | 1 | 1 | Maysville Regional | Owen County | |
| Paul Laurence Dunbar | C | 2 | | Kentucky Science Olympiad | Fayette County | |
| Prestonsburg High School | C | 1 | 1 | Invitational Only | Floyd | |
| Russell High School | C | 2 | 2 | Maysville Regional | Russell Independent Schools |
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| St Henry District High School | C | 1 | 1 | Maysville Regional | Diocese of Covington | |
| St. Albert the Great Homeschool Team | B | 1 | | Lexington Regional | Homeschool (within Jefferson County) | |
| Taylor County High School | C | 1 | | Kentucky Science Olympiad | Taylor | |
| The Gatton Academy of Mathematics and Science | C | 1 | 1 | Lexington Regional | Kentucky Board of Education | |
| Winburn Middle School | B | 1 | 1 | Kentucky Science Olympiad | Fayette | |
| Yellow Creek School Center | B | 1 | 1 | Lexington Regional | Bell | |