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| Algonquin Middle School | B | 1 | | MI Regional 07 | Chippewa Valley Schools | MI |
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| Alston Ridge Middle School | B | 3 | | Raleigh Regional | Wake County Public School System | NC |
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| Ann Arbor Skyline High School | C | 1 | 1 | MI Regional 09 | Ann Arbor Public Schools | MI | |
| Anna Middle School | B | 2 | | Western Ohio Regional Tournament | Anna Local Schools | OH | |
| Athens Drive Magnet High School | C | 3 | | Raleigh Regional Tournament | Wake County Public School System | NC |
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| Baker Middle School | B | 1 | | Oakland County - MI Region 6 | Troy School District | MI | |
| British International School of Chicago South Loop | B | 2 | | Illinois Regional | Private International School | IL | |
| British International School of Chicago South Loop | C | 2 | | Illinois State Science Olympiad | Private International School | IL | |
| Central High School | C | 2 | | Southeast Regional at PSU Abington | Philadelphia School District | PA | |
| CG Woodson High School | C | 3 | | National Ready Regional @ Mary Washington University | Fairfax County Public Schools | VA |
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| Chippewa Valley High School | C | 2 | 2 | Macomb #7 | Chippewa Valley Schools | MI |
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| Clague Middle School | B | 2 | | Michigan, Region 9 | Ann Arbor Public School | MI |
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| Cumberland Valley High School | C | 3 | | Central Region at Millersville University | Cumberland Valley School District | PA |
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| Davis Drive Middle School | B | 2 | | | Wake County Public School System | NC | |
| Dunlap High School | C | 2 | 2 | Illinois Regional | CUSD 323 | IL | |
| Durham Academy Upper School | C | 1 | | Durham | Independent School | NC | |
| Eagan High School | C | 1 | | | Rosemount-Apple Valley-Eagan ISD 196 | MN | |
| Eagle View Middle School | B | 2 | | Central Region at Millersville University | Cumberland Valley School District | PA | |
| Early College High School | C | 1 | | Akron Regional Science Olympiad (Eastern) | Canton City | OH | |
| Eisenhower High School | C | 2 | | Macomb County - MI Region 7 | Utica Community Schools | MI |
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| Flint Hill School | B | 1 | | VA | Independent | VA | |
| Flint Hill School | C | 2 | | Written Test Day (2/11) | Independent | VA |
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| Fred J. Carnage Middle School | B | 3 | | | Wake County Public Schools | NC |
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| G.A. Stetson Middle School | B | 2 | | Central East Region at Kutztown University | West Chester | PA | |
| Grand Haven High School | C | 1 | 1 | Mi Regional 12 | Grand Haven Area Public Schools | MI | |
| Green Hope High School | C | 2 | | National Ready Regional | Wake County School District | NC |
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| Greenville High School | C | 2 | 2 | Michigan Science Olympiad-R11 | Greenville Public Schools | MI | |
| Hadley E. Watts Middle School | B | 3 | | Western Ohio Regional Tournament | Centerville City Schools | OH | |
| Hillside Middle School | B | 2 | | | Northville | MI | |
| Kenston High School | C | 2 | | Northeast & Northern Ohio Regional Tournament | Kenston Local Schools | OH | |
| Keystone Academy | B | 2 | | Region 8 | National Heritage Academies Charter Schools | MI |
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| L'Anse Creuse High School North | C | 2 | 2 | REGION 7 | L'Anse Creuse Public Schools | MI | |
| Lake Braddock High School | C | 3 | | | Fairfax County Public Schools | VA | |
| Lake Central High School | C | 1 | | Valpo Regional | Lake Central School Corp. | IN | |
| Langley High School | C | 2 | 2 | University Mary Washington National Ready Regional | Fairfax County | VA | |
| Lansing HEART | C | 1 | 1 | Region 11 | Homeschool | MI |
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| Larson Middle School | B | 1 | | Oakland County - MI Region 6 | Troy | MI | |
| Legg Middle School | B | 2 | | Midwest | Coldwater Community Schools | MI |
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| Longfellow Middle School | B | 1 | | University of Mary Washington | Fairfax County Public Schools | VA |
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| Manheim Township High School | C | 1 | 1 | | Manheim Township School District | PA | |
| Meads Mill Middle School | B | 2 | | | Northville Public Schools | MI |
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| Metro Early College High School | C | 1 | 1 | Central Ohio Regional Tournament | Metro Schools | OH |
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| Mountain View MS | B | 2 | | Central | Cumberland Valley School District | PA | |
| Moxie ‘Possums | B | 1 | 1 | Winston-Salem Regional | North Carolina | NC | |
| Mt. Lebanon High School | C | 3 | | Southwest Region at PennWest California | Mt. Lebanon School District | PA | |
| New Albany High School | C | 2 | | Central Ohio Regional Tournament | New Albany Plain Local School District | OH | |
| Northville High School | C | 4 | | | Northville | MI |
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| Notre Dame Academy Toledo | C | 2 | | BGSU Northwest Ohio Regional | Notre Dame Academy | OH | |
| Novi Meadows | B | 3 | | Novi Meadows | Novi Community School District | MI | |
| Novi Middle School | B | 3 | | Oakland County - MI Region 6 | Novi Community School District | MI | |
| Ottawa Hills High School | C | 1 | | BGSU Northwest Ohio Regional | Ottawa Hills Local Schools | OH | |
| Ottawa Hills JH | B | 2 | | BGSU Northwest Ohio Regional | Ottawa Hills | OH | |
| Peirce Middle School | B | 1 | | Central East Region at Kutztown University | West Chester | PA | |
| Penncrest High School | C | 1 | | Southeast Regional at PSU Abington | Rose Tree Media | PA | |
| Pittsville High School | C | 1 | | | Pittsville School District | WI | |
| Plymouth Scholars Charter Academy | B | 1 | 1 | Region 8 | Plymouth Scholars Charter Academy | MI | |
| Rea Farms STEAM Academy | B | 2 | | North Carolina | Charlotte Mecklenburg Schools | NC |
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| Salem High School | C | 1 | 1 | Wayne Monroe Science Olympiad | Plymouth Canton Community Schools | MI | |
| Smith Middle School | B | 3 | 3 | Oakland County - MI Region 6 | Troy School District | MI | |
| South Arbor Charter Academy | B | 3 | | MI Regional 09 | National Heritage Academies | MI | |
| South Lyon High School | C | 1 | | Oakland County - MI Region 6 | South Lyon Schools | MI | |
| South Middle School (IL) | B | 3 | | Illinois Regional | Arlington Heights School District 25 | IL | |
| Thales Academy Apex 4-12 | C | 3 | 3 | Durham Regional | Wake County | NC | |
| Thales Academy Apex 4-12 | B | 2 | 2 | Durham Regional | Wake County | NC | |
| The Franciscan School | B | 1 | | Durham | Diocese of Raleigh | NC | |
| The Roeper School | B | 1 | | | Independent | MI | |
| The Shipley School B | B | 2 | 2 | Southeast Regional at PSU Abington | Lower Merion (Independent) | PA | |
| Tri-North Middle School | B | 1 | | Butler University Regional | Monroe County Community School Corporation | IN | |
| Twin Valley High School | C | 1 | | Central East Region at Kutztown University | Twin Valley School District | PA | |
| Waldron Mercy Academy | B | 1 | 1 | Southeast Regional at PSU Abington | Lower Merion School District | PA | |
| Washtenaw International High School | C | 1 | | | WEOC | MI | |
| Waynesboro High School | C | 1 | | Central Region at Millersville University | Waynesboro Area School District | PA | |
| West Springfield High School | C | 1 | | Written Test Day (2/11) | Fairfax County Public Schools | VA | |
| Western Albemarle High School | C | 1 | | Central Virginia | Albemarle County Public Schools | VA | |
| Western Reserve Academy | C | 1 | | Akron Regional Science Olympiad (Eastern) | independent | OH | |
| Zzz Test Middle School | B | 2 | | | Test District | WI | |