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| Alexander Graham Bell Elementary School | B | 3 | Illinois Regional | Chicago Public Schools | IL | |
| Almond-Bancroft High School | C | 1 | | Almond-Bancroft School District | WI | |
| Alpharetta High School | C | 2 | Georgia State - Clarkston C Regional | Fulton County | GA |
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| Alvirne High School | C | 2 | All NH Region | Hudson School District (NH) | NH | |
| Archimedean Middle Conservatory | B | 3 | Southeast Regional at Florida Atlantic University | Miami-Dade | FL | |
| Baldwin-Woodville High School | C | 2 | WI Region 1 | Baldwin-Woodville Area School District | WI | |
| Chandler Woods Charter Academy | B | 1 | Mi Regional 12 | NHA Charter | MI | |
| Cornerstone Charter Academy | C | 1 | | Guilford County | NC | |
| Delano High School | C | 2 | Minnesota Science Olympiad | Delano Public Schools | MN | |
| Evansville High School | C | | Wisconsin Science Olympiad - Edgewood Regional | Evansville Community School District | WI | |
| Faribault High School | C | 1 | Minnesota Science Olympiad | Minnesota ISD #656 | MN | |
| Fulton County Schools Innovation Academy | C | 3 | Georgia State - Clarkston C Regional | Fulton County Schools | GA | |
| Garvey Intermediate | B | | SoCal Joint Regional | Garvey School District | CA |
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| Irvington High School | C | 2 | Bay Area Science Olympiad | Fremont USD | CA | |
| Jeffrey Trail Middle School | B | 2 | SoCal Joint Regional | Irvine Unified School District | CA | |
| John Jay High School East Fishkill | C | 3 | New York - Mid-Hudson Region | Wappingers Central School District | NY | |
| John Marshall High School | C | 2 | Minnesota Science Olympiad | Rochester Public Schools | MN | |
| Kennedy MS | B | 3 | | Cupertino Union School District | CA |
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| Lake Braddock High School | C | 3 | | Fairfax County Public Schools | VA | |
| Lakeland Union High School | C | 1 | Wisconsin Science Olympiad | Lakeland School District | WI | |
| Lakeville North | C | | Minnesota Science Olympiad | Independent School District 194 | MN |
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| Lakeville North | C | 2 | | Lakeville ISD 194 | MN | |
| Lincoln SFP | C | 1 | NE Regional | Lincoln Public Schools | NE | |
| Medford Area Middle School | B | 2 | | Medford Area Public Schools | WI | |
| Medford Area Senior High | C | 2 | WI Region 1 | Medford Area Public School District | WI |
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| Mentor High School | C | 2 | Northeast & Northern Ohio Regional Tournament | Mentor Public Schools | OH | |
| Mount Vernon High School | C | 1 | Iowa Science Olympiad | Mount Vernon Community School District | IA | |
| Mount Vernon Middle School | B | 1 | Iowa Science Olympiad | Mount Vernon Community School District | IA | |
| Mountain Green Middle School | B | 2 | Utah Science Olympiad | Morgan | UT | |
| Osceola Science Charter School | B | 2 | Eastern Regional at Embry Riddle Aeronautical University | The School District of Osceola County | FL |
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| Piedmont Open IB Middle School | B | 2 | | Charlotte-Mecklenburg Schools | NC |
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| Pilgrim Park Middle School | B | | | Elmbrook Schools | WI | |
| Rachel Carson Middle School | B | 3 | Written Test Day (2/11) | Fairfax County | VA | |
| Scarsdale High School | C | 4 | Lower Hudson VAlle | Scarsdale UFSD | NY | |
| Seven Lakes Junior High | B | 2 | Gulf Coast Regional | Katy ISD | TX | |
| Sierra Vista Middle School | B | 4 | | IUSD | CA |
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| Solon High School | C | 1 | Northeast & Northern Ohio Regional Tournament | Solon City Schools | OH | |
| The College Preparatory School | C | 2 | | Oakland USD | CA | |
| Timberline Middle School | B | 1 | Regional - Southwest (Division B) | Lake Washington School District | WA |
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| West Bend High School | C | 1 | WI Region 1 | West Bend School District | WI | |