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| Bala Cynwyd Middle School | B | 2 | Southeast Regional at PSU Abington | Lower Merion School District | PA | |
| BASIS Austin | B | 1 | | BASIS Texas | TX | |
| Basis Cedar Park | B | 4 | UT Austin | Charter | TX |
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| Campbell MIddle School | B | 1 | | Cobb County | GA | |
| Chaboya MS | B | 2 | | Evergreen School District | CA |
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| Challenger School- Ardenwood | B | 1 | | Challenger School | CA | |
| Clarksville Middle School | B | 2 | | Howard County Public Schools | MD | |
| Community MS | B | 2 | New Jersey Science Olympiad | West Windsor Plainsboro Regional School District | NJ |
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| Discovery Peak Charter School | B | 1 | Alaska Science Olympiad | Fairbanks North Star Borough School District | AK | |
| Dwight-Englewood School (MS) | B | 1 | New Jersey Science Olympiad | Private | NJ | |
| Fugett Middle School | B | 2 | Central East Region at Kutztown University | West Chester Area School District | PA | |
| G.A. Stetson Middle School | B | 2 | Southeast Regional at PSU Abington | West Chester | PA | |
| Garnet Valley Middle School | B | 3 | Southeast Regional at PSU Abington | Garnet Valley | PA |
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| Henry E. Huntington Middle School | B | 2 | | San Marino Unified | CA | |
| IS98 Bay Academy | B | 2 | | District 21 | NY |
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| J. Michael Lunsford Middle School | B | 3 | Written Test Day (2/11) | Loudoun County Public Schools | VA |
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| Jeffrey Trail Middle School | B | 4 | SoCal Joint Regional | Irvine Unified School District | CA | |
| Kealing Middle School | B | 3 | UT Austin | Austin ISD | TX | |
| Kennedy MS | B | 6 | | Cupertino Union School District | CA |
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| Longfellow Middle School | B | 2 | | Fairfax County Public Schools | VA |
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| Louise Archer ES | B | 1 | Written Test Day (2/11) | Fairfax County Public School | VA |
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| Orange County School of the Arts | B | 2 | Orange County | Independent Charter | CA | |
| Peirce Middle School | B | 1 | Central East Region at Kutztown University | West Chester Areas School District | PA | |
| Pleasant Ridge Middle School | B | 2 | Northeast Regional | Blue Valley School District USD 229 | KS |
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| Robert C. Fisler K-8 | B | 2 | SoCal Joint Regional | Fullerton School District | CA | |
| Robert E Bell Middle School | B | 2 | | Chappaqua Central School District | NY |
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| Sartartia Middle School | B | 2 | San Antonio Regional | Fort Bend ISD | TX | |
| Sierra Vista Middle School | B | 5 | | IUSD | CA |
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| Springhouse Middle School | B | 2 | Central East Region at Kutztown University | Parkland | PA |
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| Springton Lake Middle School | B | 1 | Southeast Regional at PSU Abington | Rose Tree Media | PA | |
| Stoller Middle School | B | 2 | Oregon Science Olympiad | Beaverton School District | OR |
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| Timberline Middle School | B | 6 | Regional - Southwest (Division B) | Lake Washington School District | WA |
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| Venado Middle School | B | 2 | Southern California | Irvine Unified School District | CA |
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| Vickery Creek Middle School | B | 1 | Jackson Co B Regional | Forsyth County | GA |
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| Wayne Highlands Middle School | B | 1 | Northeast Region at PSU Wilkes-Barre | Wayne Highlands School District | PA | |
| Windemere Ranch Middle School | B | 4 | | San Ramon Valley USD | CA |
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| Wydown Middle School | B | 1 | Region 6 B | School District of Clayton | MO | |