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| Baker Middle School | B | 2 | Oakland County - MI Region 6 | Troy School District | MI | |
| BASIS Cedar Park | B | 2 | Texas UT Austin | Charter | TX |
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| Beachwood Middle School | B | 2 | Northeast & Northern Ohio Regional Tournament | Beachwood | OH | |
| Chardon Middle School | B | 2 | Northeast & Northern Ohio Regional Tournament | Chardon | OH | |
| Copley Middle School | B | 1 | Akron Regional Science Olympiad (Eastern) | Copley-Fairlawn City Schools | OH | |
| Daniel Wright Junior High School | B | 2 | Illinois Regional | Lincolnshire-Prairie View District 103 | IL | |
| East Middle School - Plymouth | B | 1 | Wayne Monroe Science Olympiad | Plymouth-Canton Community Schools | MI | |
| Hadley E Watts Middle School | B | 2 | Western Ohio Regional Tournament | Centerville City Schools | OH |
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| Hillside Middle School | B | 2 | | Northville | MI | |
| Hudson Middle School | B | 2 | Akron Regional Science Olympiad (Eastern) | Hudson City Schools | OH |
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| Kenston Middle School | B | 2 | Northeast & Northern Ohio Regional Tournament | Kenston Local School District | OH | |
| Marie Murphy School | B | 1 | Illinois Regional | Avoca School District #37 | IL | |
| Mason Intermediate | B | 1 | Southwestern Ohio Science Olympiad | Mason City Schools | OH | |
| Meads Mill Middle School | B | 2 | | Northville Public Schools | MI |
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| Memorial Middle School | B | 2 | Northeast & Northern Ohio Regional Tournament | Mentor Public Schools | OH | |
| Science & Arts Academy | B | 1 | Oakton (TBD) | Independent school | IL |
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| Shore Middle School | B | 2 | Northeast & Northern Ohio Regional Tournament | Mentor | OH | |
| Slauson Middle School | B | 2 | | Ann Arbor Public Schools | MI |
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| Solon Middle School | B | 2 | Northeast & Northern Ohio Regional Tournament | Solon City Schools | OH |
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| Westlake MS | B | 2 | Northeast & Northern Ohio Regional Tournament | Westlake City School District | OH |
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