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| AAH (Austin Area Homeschoolers) | B | 1 | 1 | University of Texas Regional | Homeschool | TX | |
| Ames Middle School | B | 2 | 2 | Iowa State | Ames Community School District | IA | |
| Arbor Hills Junior High | B | 1 | 1 | OHSO West Regional | Sylvania Schools | OH | |
| Asotin MS | B | 1 | 1 | WSO 2020-21 Statewide Regional B-C Tournament | Asotin-Anatone School District | WA | |
| Beachwood Middle School | B | 2 | 2 | Northeast Ohio Regional Tournament | Beachwood | OH | |
| Chaboya MS | B | 1 | 1 | | Evergreen School District | CA | |
| Chardon Middle School | B | 1 | 1 | Northeast Ohio Regional Tournament | Chardon Local Schools | OH | |
| Christensen Middle School | B | 3 | 3 | Bay Area Science Olympiad | Livermore Valley Joint Unified School District | CA |
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| Community Middle School | B | 2 | 2 | | West Windsor Plainsboro School District | NJ | |
| Granville Middle School | B | 2 | 2 | Western Ohio Regional Tournament | Granville Exempted Village School District | OH | |
| Harmony School of Excellence - Houston | B | 2 | 2 | San Antonio Regional | Harmony Public Schools | TX | |
| Highlands Intermediate School | B | 2 | 2 | HSSO Div B Regional | Leeward District | HI | |
| Hillside Middle School | B | 1 | 1 | MI Regional 8 | Northville Public Schools | MI | |
| Hudson Middle School | B | 3 | 3 | OHSO West Regional | Hudson City Schools | OH |
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| Iolani School | B | 2 | 2 | HSSO Div B Regional | Oahu | HI | |
| Jeffrey Trail Middle School | B | 1 | 1 | SoCal Joint Regional | Irvine Unified School District | CA | |
| Kealing Middle School | B | 1 | 1 | University of Texas Regional | Austin ISD | TX | |
| Kennedy MS | B | 2 | 2 | Santa Clara County Regional Science Olympiad | Cupertino Union School District | CA |
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| Kenston Middle School | B | 3 | 3 | Northeast Ohio Regional Tournament | Kenston Local Schools | OH |
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| Marshall Middle School | B | 3 | 3 | San Diego Regional Tournament 2 | San Diego Unified School District | CA | |
| McCord Junior High | B | 1 | 1 | OHSO West Regional | Sylvania Schools | OH | |
| Meads Mill Middle School | B | 1 | 1 | MI Regional 08 | Northville Public Schools | MI |
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| Memorial Middle School | B | 2 | 2 | Northeast Ohio Regional Tournament | Mentor Public Schools | OH | |
| Mesa Robles School | B | 2 | 2 | Los Angeles - South | Hacienda La Puente USD | CA | |
| Metrolina Regional Scholars Academy | B | 1 | | | Metrolina Regional Scholars Academy NCES District ID: 3700107 | NC | |
| Meyzeek Middle School | B | 3 | 3 | Louisville Regional | Jefferson county Public Schools | KY |
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| Miller MS | B | 2 | 2 | | Cupertino Union School District | CA | |
| Mount Vernon Middle School | B | 1 | 1 | Iowa State | Mount Vernon Community School District | IA | |
| North Austin Science Alliance | B | 1 | 1 | University of Texas Regional | Homeschool Team | TX | |
| Osceola Science Charter School | B | 2 | | | The School District of Osceola County | FL |
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| Pacific Trails Middle School | B | 3 | 3 | San Diego | San Dieguito Union High School District | CA |
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| Piedmont IB Middle School | B | 2 | 2 | Charlotte | Charlotte Mecklenburg Schools | NC |
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| Raymond Park Intermediate and Middle School | B | 1 | 1 | INSO Satellite Regional #1 | MSD Warren Township | IN | |
| Robert C. Fisler K-8 | B | 1 | 1 | SoCal Joint Regional | Fullerton School District | CA |
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| Seminole Science Charter School | B | 1 | 1 | Eastern Regional Satellite SO | Seminole | FL | |
| Shady Side Academy Middle School | B | 2 | 2 | Mini Satellite Hybrid SO Regional | Independent | PA | |
| Shore Middle School | B | 2 | 2 | Northeast Ohio Regional Tournament | Mentor | OH | |
| Sierra Vista Middle School - IUSD | B | 2 | | SoCal Joint Regional | IUSD | CA | |
| Smith Middle School | B | 1 | 1 | MI Regional 06 | Troy School District | MI | |
| Solon Middle School | B | 2 | 2 | Northeast Ohio Regional Tournament | Solon City School District | OH | |
| Springfield Middle School | B | 1 | 1 | OHSO West Regional | Springfield Local | OH | |
| Springhouse Middle School | B | 2 | 2 | Mini Satellite Hybrid SO Regional | Parkland | PA | |
| St. Rose Perrysburg | B | 1 | 1 | OHSO West Regional | Diocese of Toledo | OH | |
| Timberline Middle School | B | 3 | 3 | WSO 2021 Statewide Regional B/C | Lake Washington School District | WA | |
| Timberstone Junior High | B | 2 | 2 | OHSO West Regional | Sylvania Schools | OH | |
| Westlake LBMS | B | 4 | 4 | Northeast Ohio Regional Tournament | Westlake City School District | OH |
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| Winston Churchill Middle School | B | 3 | 3 | Sacramento Regional Science Olympiad | San Juan Unified School District | CA | |
| Wyoming Middle School | B | 2 | | Northeast Ohio Regional Tournament | Wyoming City Schools | OH | |