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| Bearden Middle School | B | 1 | East Tennessee State University Regional | Knox County School | TN |
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| Boulan Park Middle School | B | 3 | Oakland County - MI Region 6 | Troy School District | MI | |
| Canal Winchester Middle School | B | 1 | Central Ohio Regional Tournament | Canal Winchester Local Schools | OH | |
| Chardon Middle School | B | 2 | Northeast & Northern Ohio Regional Tournament | Chardon Local Schools | OH | |
| Daniel Wright Junior High School | B | 2 | Illinois Regional | Lincolnshire-Prairie View District 103 | IL | |
| Hillside Middle School | B | 2 | | Northville | MI | |
| Hudson Middle School | B | 3 | Northern Ohio Regional | Hudson City Schools | OH |
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| Incarnate Word Academy | B | 1 | Northeast & Northern Ohio Regional Tournament | PARMA CSD | OH |
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| Kenston Middle School | B | 2 | OH Practice R1 | Kenston Local School District | OH | |
| Magsig Middle School | B | 2 | Western Ohio Regional Tournament | Centerville City Schools | OH |
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| Marie Murphy School | B | 1 | Illinois Regional | Avoca School District #37 | IL | |
| Mason Middle School | B | 2 | Southwestern Ohio Science Olympiad | Mason City Schools | OH |
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| Meads Mill Middle School | B | 2 | | Northville Public Schools | MI |
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| Memorial Middle School | B | 2 | Northeast & Northern Ohio Regional Tournament | Mentor Public Schools | OH | |
| North Pocono Middle School | B | 2 | Northeast Region at PSU Wilkes-Barre | North Pocono School District | PA | |
| Novi Middle School | B | 1 | Oakland County - MI Region 6 | Novi Community School District | MI | |
| Rocky River Middle School | B | 2 | Northeast & Northern Ohio Regional Tournament | Rocky River City School District | OH | |
| Science & Arts Academy | B | 1 | | Independent school | IL |
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| Shore Middle School | B | 2 | Northeast & Northern Ohio Regional Tournament | Mentor | OH | |
| Sierra Vista Middle School | B | 3 | Orange County | IUSD | CA |
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| Slauson Middle School | B | 2 | | Ann Arbor Public Schools | MI |
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| Solon Middle School | B | 2 | Northern Ohio Regional | Solon City Schools | OH | |
| Springhouse Middle School | B | 2 | | Parkland | PA |
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| Walnut Hills | B | 1 | Cincinnati | Cincinnati Public | OH | |
| Westlake MS | B | 3 | Northern Ohio Regional | Westlake City School District | OH |
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| Wilbur Wright Middle School | B | 1 | Valpo Regional | School Town of Munster | IN |
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