| | | | | | |
| Alexander W. Dreyfoos, Jr. School of the Arts | C | 2 | 2 | Southeast Regional at Florida Atlantic University | School District of Palm Beach County | |
| All Saints Academy | B | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Independent | |
| Allen D Nease High School | C | 2 | 2 | Eastern Regional at Embry Riddle Aeronautical University | St Johns | |
| American Heritage Plantation | B | 2 | 2 | Southeast Regional at Florida Atlantic University | Broward | |
| American Heritage Plantation | C | 3 | 3 | Southeast Regional at Florida Atlantic University | Broward | |
| American Heritage School | C | 1 | 2 | Southern Regional at University of Miami | Palm Beach | |
| Archbishop McCarthy High School | C | 2 | | Southwest Regional at Canterbury School | Archdiocese of Miami | |
| Archimedean Middle Conservatory | B | 2 | 2 | Southeast Regional at Florida Atlantic University | Miami-Dade | |
| Archimedean Upper Conservatory | C | 4 | 4 | Southeast Regional at Florida Atlantic University | Miami-Dade | |
| Bartow International Baccalaureate | C | 2 | 2 | Western Regional at Hillsborough College, Brandon Campus | Polk | |
| Bartram Trail High School | C | 2 | 2 | Eastern Regional at Embry Riddle Aeronautical University | St. Johns | |
| Beulah Academy of Science | B | 1 | | Virtual Regional - State Qualifier | Escambia County | |
| Blake High | C | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough County Public Schools | |
| Boca Raton Community High School | C | 4 | 4 | Southeast Regional at Florida Atlantic University | Palm Beach | |
| Broward Math and Science Schools | B | 1 | 1 | Southeast Regional at Florida Atlantic University | Broward | |
| Buchholz High School | C | 2 | 1 | Northern Regional at Buchholtz High School | Alachua County Public Schools | |
| Buchholz High School | B | 1 | | Northern Regional at Buchholtz High School | Alachua County Public Schools | |
| Burns Scitech Charter High School | C | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Caloosa Middle School | B | 1 | 1 | Southwest Regional at Canterbury School | School District of Lee County | |
| Calvary Chapel Academy | C | 1 | 1 | Southeast Regional at Florida Atlantic University | Private School | |
| Canterbury School | C | 2 | 2 | Southwest Regional at Canterbury School | Private | |
| Cape Coral High School | C | 1 | | Southwest Regional at Canterbury School | Lee County | |
| Carwise Middle School | B | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Pinellas | |
| Cedar Key School | B | 1 | | Central Regional at Olympia High School (Orlando) | Levy County | |
| Celebration High School | C | 2 | 2 | Central Regional at Olympia High School (Orlando) | Osceola County Public Schools | |
| Challenger Middle School | B | 1 | 1 | Southwest Regional at Canterbury School | School District of Lee County | |
| Chaminade-Madonna College Preparatory | C | 1 | 1 | Southeast Regional at Florida Atlantic University | Private | |
| Community School of Naples | B | 1 | | Southwest Regional at Canterbury School | Collier County | |
| Coral Glades High School | C | 2 | 2 | Southeast Regional at Florida Atlantic University | Broward County Public Schools | |
| Country Day World School | B | 2 | 2 | Western Regional at Hillsborough College, Brandon Campus | Private | |
| Creekside Middle School | B | 1 | | Eastern Regional at Embry Riddle Aeronautical University | Volusia County | |
| Crystal Lake Middle School | B | 2 | | Southern Regional at University of Miami | Broward County | |
| DeLand High School | C | 3 | 3 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Deltona High School | C | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia County Schools | |
| Discovery Academy of Science | B | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| Discovery Academy of Science | A | 2 | 2 | Florida Elementary Science Olympiad | Pinellas County Schools | |
| Doral Academy High School | C | 2 | | Southern Regional at University of Miami | Miami-Dade | |
| Dr Phillips High School | C | 1 | 1 | Central Regional at Olympia High School (Orlando) | Orange County | |
| Dr. Kiran C Patel High School | C | 2 | 2 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough | |
| East Lake High school | C | 2 | 2 | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| East Lake Middle School Academy of Engineering | B | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| Everglades High School | C | 3 | 3 | Southern Regional at University of Miami | Broward | |
| Fairview Middle School | B | 4 | 4 | Northern Regional at Buchholtz High School | Leon County |
|
| Florida Atlantic University High School | C | 2 | 2 | Southeast Regional at Florida Atlantic University | FAU Schools | |
| Florida State University Schools | C | 1 | 1 | Northern Regional at Buchholtz High School | Florida State University Schools | |
| Fort Myers High School | C | 3 | 3 | Southwest Regional at Canterbury School | School District of Lee County | |
| Galaxy Middle School | B | 3 | 4 | Eastern Regional at Embry Riddle Aeronautical University | Volusia |
|
| Hagerty High School | C | 1 | 1 | Central Regional at Olympia High School (Orlando) | Seminole County | |
| Hialeah Gardens High School | C | 1 | 1 | Southern Regional at University of Miami | Miami-Dade County Public Schools | |
| Hillsborough High School | C | 1 | | Western Regional at Hillsborough College, Brandon Campus | Hillsborough County | |
| Horizon High School | C | 1 | 1 | Central Regional at Olympia High School (Orlando) | Orange | |
| iBuild Academy, Inc | B | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Imagine School at Land O' Lakes | B | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Pasco | |
| International Studies Charter High Schools | C | 1 | | Southern Regional at University of Miami | Miami Dade County Public Schools | |
| James S. Rickards High School | C | 6 | 6 | Northern Regional at Buchholtz High School | Leon County | |
| Joe. E, Newsome high School | C | 2 | 2 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough county public Schools | |
| Lake Highland Preparatory School | B | 2 | 2 | Central Regional at Olympia High School (Orlando) | Independent | |
| Lake Nona High School | C | 2 | 2 | Central Regional at Olympia High School (Orlando) | Orange | |
| Land O Lakes High School | C | 3 | 3 | Western Regional at Hillsborough College, Brandon Campus | Pasco County School District | |
| Largo High School | C | 3 | 3 | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| Lawton Chiles High School | C | 2 | 2 | Northern Regional at Buchholtz High School | Leon County | |
| Liberty Pines Academy | B | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Saint Johns County | |
| Maclay School | C | 1 | 1 | Northern Regional at Buchholtz High School | Private | |
| Mainland High School | C | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Mary Help of Christians | B | 1 | 1 | Southeast Regional at Florida Atlantic University | Archdiocese of Miami | |
| Matanzas High School | C | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Flagler County School District | |
| Middleton High School | C | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough county public schools | |
| Morgan Fitzgerald Middle School | B | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Pinellas | |
| Navarre Beach Marine Science Station | C | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Santa Rosa | |
| Navarre High School - Florida | C | 4 | 4 | Eastern Regional at Embry Riddle Aeronautical University | Santa Rosa | |
| New Springs Schools | B | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough county schools | |
| North Fort Myers High A | C | 1 | 1 | Southwest Regional at Canterbury School | Lee County Schools |
|
| Oak Hall School | C | 1 | 1 | Northern Regional at Buchholtz High School | Oak Hall School | |
| OLSEN MIDDLE SCHOOL | B | 1 | 1 | Southern Regional at University of Miami | BROWARD COUNTY PUBLIC SCHOOLS | |
| Olympia High School | C | 6 | 6 | Central Regional at Olympia High School (Orlando) | Orange County Public Schools | |
| Olympic Heights High School | C | 2 | 2 | Southeast Regional at Florida Atlantic University | Palm Beach School District | |
| Orlando Gifted Academy | B | 1 | 1 | Central Regional at Olympia High School (Orlando) | Orange County Public Schools | |
| Orlando Science High School | C | 6 | 6 | Central Regional at Olympia High School (Orlando) | Orange | |
| Orlando Science Middle School | B | 5 | 5 | Central Regional at Olympia High School (Orlando) | Orange |
|
| Osceola High School | C | 4 | 4 | Central Regional at Olympia High School (Orlando) | School District of Osceola County | |
| Osceola Science Charter School | B | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | The School District of Osceola County |
|
| Palm Beach Central High School | C | 2 | 2 | Southeast Regional at Florida Atlantic University | Palm Beach | |
| Palm Beach Day Academy | B | 1 | | Southeast Regional at Florida Atlantic University | Private | |
| Palm Harbor University High School | C | 3 | 3 | Western Regional at Hillsborough College, Brandon Campus | Pinellas | |
| Pembroke Pines Charter Academic Village | B | 3 | 3 | Southeast Regional at Florida Atlantic University | Broward | |
| Pembroke Pines Charter Central Middle School | B | 7 | 7 | Southeast Regional at Florida Atlantic University | Broward | |
| Pembroke Pines Charter High School | C | 5 | 4 | Southeast Regional at Florida Atlantic University | Broward | |
| Pensacola High School | C | 1 | 1 | Northern Regional at Buchholtz High School | Escambia |
|
| Pine Ridge High School | C | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Pine View School | B | 2 | 2 | Western Regional at Hillsborough College, Brandon Campus | Sarasota | |
| Pine View School | C | 2 | 2 | Western Regional at Hillsborough College, Brandon Campus | Sarasota | |
| Plant City High School | C | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough | |
| Randall Middle School | B | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough County Public Schools | |
| River City Science Academy Elementary | A | 1 | | Florida Elementary Science Olympiad | Duval | |
| River city Science Academy Innovation | B | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | DUVAL | |
| River City Science Academy Intracoastal | B | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Duval | |
| River City Science Academy M-H | B | 4 | 4 | Eastern Regional at Embry Riddle Aeronautical University | DUVAL |
|
| River City Science Academy M-H | C | 2 | 2 | Eastern Regional at Embry Riddle Aeronautical University | DUVAL |
|
| River City Science Academy Mandarin | B | 2 | 2 | Northern Regional at Buchholtz High School | DUVAL |
|
| River City Science Academy Southeast | B | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Duval | |
| Riverview High School | C | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Sarasota | |
| Roberto Clemente Middle School | B | 1 | 1 | Central Regional at Olympia High School (Orlando) | Orange County | |
| Saint Andrew Catholic School | B | 1 | 1 | Southeast Regional at Florida Atlantic University | Archdiocese of Miami | |
| School for Advanced Studies- South | C | 2 | | Southern Regional at University of Miami | Miami-Dade County Public Schools | |
| School for Advanced Studies- West | C | 1 | | Southern Regional at University of Miami | Miami-Dade County Public Schools | |
| School for Advanced Studies-West | C | 1 | | Southern Regional at University of Miami | Miami Dade Public County Schools | |
| Seabreeze High School | C | 2 | 2 | Eastern Regional at Embry Riddle Aeronautical University | Volusia County Schools | |
| Seminole High School | C | 2 | 2 | Central Regional at Olympia High School (Orlando) | Seminole County Public Schools | |
| Seminole Science Charter School | B | 2 | 2 | Central Regional at Olympia High School (Orlando) | Seminole |
|
| silver Sands Middle School | B | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Somerset Academy High School | C | 2 | 2 | Southern Regional at University of Miami | Broward | |
| Somerset College Prep Academy | C | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | St. Lucie | |
| South Broward High School | C | 2 | 2 | Southern Regional at University of Miami | Broward Schools | |
| South Walton Academy | C | 1 | 1 | Southwest Regional at Canterbury School | South Walton | |
| South Walton High School | C | 1 | | Western Regional at Hillsborough College, Brandon Campus | Walton County School District | |
| Southeast High School | C | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Manatee School District | |
| Spanish River High School | C | 2 | 2 | Southeast Regional at Florida Atlantic University | Palm Beach County School District | |
| Spruce Creek High School | C | 3 | 3 | Eastern Regional at Embry Riddle Aeronautical University | Volusia County School District |
|
| St. Bonaventure Catholic School | B | 1 | 1 | Southern Regional at University of Miami | Archdiocese of Miami | |
| St. Johns Country Day School | B | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Private | |
| St. Thomas Aquinas High School | C | 3 | 3 | Southern Regional at University of Miami | Archdiocese of Miami | |
| St.Petersburg High School | C | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| Stanton College Preparatory | C | 3 | 3 | Northern Regional at Buchholtz High School | Duval County Public Schools | |
| Strawberry Crest High School | C | 2 | | Western Regional at Hillsborough College, Brandon Campus | Hillsborough County | |
| Suncoast Community High School | C | 2 | 2 | Southeast Regional at Florida Atlantic University | Palm Beach School District | |
| Swift Creek Middle School | B | 1 | 1 | Northern Regional at Buchholtz High School | Leon | |
| Tallahasse School of Math and Science | B | 1 | 1 | Northern Regional at Buchholtz High School | Leon County | |
| Tampa Catholic High School | C | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Diocese of Saint Petersburg | |
| Tampa Preparatory School | C | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough | |
| Tampa Preparatory School | B | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough | |
| The King's Academy | C | 1 | 1 | Southeast Regional at Florida Atlantic University | TKA-FL | |
| The Villages Charter Middle School | B | 1 | 1 | Central Regional at Olympia High School (Orlando) | Sumter | |
| THINK - Tampa Homeschoolers Instructing and Nurturing Kids | B | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough | |
| Timber Creek High School | C | 2 | 2 | Central Regional at Olympia High School (Orlando) | Orange County Public Schools | |
| Trinity Preparatory School | C | 3 | 3 | Central Regional at Olympia High School (Orlando) | Independent School | |
| Universal Academy of Florida | C | 1 | | Western Regional at Hillsborough College, Brandon Campus | Hillsborough | |
| University High School | C | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia County Schools | |
| University High School | C | 1 | 1 | Eastern Regional at Embry Riddle Aeronautical University | Orange | |
| Velocity Raptors (Homeschool Team) | A | 1 | | Florida Elementary Science Olympiad | Palm Beach County | |
| Velocity Raptors (Homeschool Team) | B | 1 | 1 | Southeast Regional at Florida Atlantic University | Palm Beach County | |
| Villages High School | C | 2 | 2 | Central Regional at Olympia High School (Orlando) | Sumter | |
| West Broward High School | C | 3 | 3 | Southeast Regional at Florida Atlantic University | Broward County | |
| Windermere High School | C | 1 | | Central Regional at Olympia High School (Orlando) | Orange county | |
| Winter Park High School | C | 1 | 1 | Central Regional at Olympia High School (Orlando) | Orange County Public Schools | |
| Wiregrass Ranch High School | C | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Pasco | |