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| Academy at the Farm | C | 1 | | Western Regional at Hillsborough College, Brandon Campus | Pasco County School District | |
| Academy at the Farm | B | 1 | | Western Regional at Hillsborough College, Brandon Campus | Pasco County School District | |
| Academy at the Lakes | C | 1 | | Western Regional at Hillsborough College, Brandon Campus | Pasco | |
| Allen D Nease High School | C | 3 | | Northern Regional at The University of Florida | St Johns | |
| American Heritage Plantation | C | 2 | 2 | Southeastern Regional at Florida Atlantic University | Broward | |
| American Heritage Plantation | B | 2 | 2 | Southeastern Regional at Florida Atlantic University | Broward | |
| American Heritage School | C | 1 | | Southern Regional at University of Miami | Palm Beach | |
| Archimedean Middle Conservatory | B | 3 | 3 | Southeastern Regional at Florida Atlantic University | Miami-Dade | |
| Archimedean Upper Conservatory | C | 4 | 4 | Southeastern Regional at Florida Atlantic University | Miami-Dade | |
| Aubrey Rogers Highschool | C | 2 | | Southwestern Regional at Canterbury School | Collier County | |
| Bartow International Baccalaureate | C | 2 | 2 | Western Regional at Hillsborough College, Brandon Campus | Polk | |
| Beachside High School | C | 1 | | Northern Regional at The University of Florida | St. Johns County School District | |
| Blake High | C | 2 | | Western Regional at Hillsborough College, Brandon Campus | Hillsborough County Public Schools | |
| Boca Raton Community High School | C | 4 | 4 | Southeastern Regional at Florida Atlantic University | Palm Beach | |
| Buchholz High School | C | 1 | 1 | Northern Regional at The University of Florida | Alachua County | |
| Buchholz High School | C | 1 | | Northern Regional at The University of Florida | Alachua County Public Schools | |
| Burns Scitech Charter High School | C | 1 | | Eastern Regional at Stetson University | Volusia | |
| Burns Scitech Charter Middle School | B | 1 | | Eastern Regional at Stetson University | Volusia | |
| Caloosa Middle School | B | 1 | | Southwestern Regional at Canterbury School | School District of Lee County | |
| Calvary Chapel Academy | C | 2 | 2 | Eastern Regional at Stetson University | Private School | |
| Calvary Chapel Academy | B | 1 | 1 | Eastern Regional at Stetson University | Private School | |
| Carwise Middle School | B | 1 | | Western Regional at Hillsborough College, Brandon Campus | Pinellas | |
| Celebration High School | C | 2 | | Central Regional at Osceola High School | Osceola County Public Schools | |
| Chaminade-Madonna College Preparatory | C | 3 | | Southeastern Regional at Florida Atlantic University | Private | |
| City of Pembroke Pines Charter Middle School WEST Campus | B | 1 | | Southeastern Regional at Florida Atlantic University | Broward | |
| Clark Advanced Learning Center | C | 1 | 1 | Southeastern Regional at Florida Atlantic University | Martin County School District | |
| Clearwater Central Catholic High School | C | 1 | | Western Regional at Hillsborough College, Brandon Campus | DOSP | |
| David C Hinson Middle School | B | 1 | 1 | Eastern Regional at Stetson University | Volusia County | |
| Deltona High School | C | 1 | 1 | Eastern Regional at Stetson University | Volusia County | |
| Discovery Academy of Science | B | 1 | | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| Durant High School | C | 1 | | Western Regional at Hillsborough College, Brandon Campus | Hillsborough County | |
| East Lake High school | C | 2 | | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| East Lake Middle School Academy of Engineering | B | 1 | | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| Everglades High School | C | 1 | | Southern Regional at University of Miami | Broward | |
| Fairview Middle School | B | 4 | 4 | Northern Regional at The University of Florida | Leon County |
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| Florida Atlantic University High School | C | 4 | 4 | Southeastern Regional at Florida Atlantic University | FAU Schools | |
| Galaxy Middle School | B | 3 | 3 | Eastern Regional at Stetson University | Volusia |
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| Gifford Middle School | B | 1 | 1 | Southeastern Regional at Florida Atlantic University | Indian River | |
| Horizon High School | C | 1 | | Central Regional at Osceola High School | Orange | |
| iBuild Academy | B | 1 | 1 | Eastern Regional at Stetson University | Volusia | |
| iBuild Academy | C | 1 | 1 | Eastern Regional at Stetson University | Volusia | |
| Innovation High School | C | 2 | | Central Regional at Osceola High School | Orange | |
| James S. Rickards High School | C | 6 | 6 | Northern Regional at The University of Florida | Leon County | |
| Julia Landon College Prepatory and Leadership Development Middle School | B | 2 | 2 | Northern Regional at The University of Florida | Duval County Public Schools | |
| Lake Highland Preparatory School | B | 1 | 1 | Central Regional at Osceola High School | Independent | |
| Lake Nona High School | C | 2 | 2 | Central Regional at Osceola High School | Orange | |
| Land O Lakes High School | C | 3 | | Western Regional at Hillsborough College, Brandon Campus | Pasco County School District | |
| Largo High School | C | 2 | | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| Maclay School | C | 1 | 1 | Northern Regional at The University of Florida | Private | |
| Magnet Innovation Center | C | 2 | 2 | Northern Regional at The University of Florida | Walton District Schools | |
| Marjory Stoneman Douglas High School | C | 1 | | Southeastern Regional at Florida Atlantic University | Broward County | |
| Mary Help of Christians | B | 1 | 1 | Southeastern Regional at Florida Atlantic University | Archdiocese of Miami | |
| Mater Academy Narcoossee | C | 1 | | Central Regional at Osceola High School | Orange County | |
| Middleton High School | C | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough county public schools | |
| Naples High School | C | 1 | | Southwestern Regional at Canterbury School | Collier County Public Schools | |
| Navarre High School - Florida | C | 5 | 5 | Central Regional at Osceola High School | Santa Rosa | |
| North Fort Myers High | C | 2 | 2 | Southwestern Regional at Canterbury School | Lee County Schools |
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| Olympia High School | C | 8 | 8 | Central Regional at Osceola High School | Orange County Public Schools | |
| Orlando Gifted Academy | B | 1 | 1 | Central Regional at Osceola High School | Orange County Public Schools | |
| Orlando Science Charter School East | B | 1 | | Central Regional at Osceola High School | Orange | |
| Orlando Science High School | C | 5 | 5 | Central Regional at Osceola High School | Orange | |
| Orlando Science Middle School | B | 4 | 4 | Central Regional at Osceola High School | Orange |
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| Osceola High School | C | 4 | 4 | Central Regional at Osceola High School | School District of Osceola County | |
| Osceola Science Charter School | B | 1 | 1 | Central Regional at Osceola High School | Osceola District | |
| Our Lady of Lourdes Academy | C | 1 | | Southern Regional at University of Miami | Archdiocese of Miami | |
| Pembroke Pines Charter Academic Village | B | 5 | 5 | Southeastern Regional at Florida Atlantic University | Broward | |
| Pembroke Pines Charter Central Middle School | B | 5 | 5 | Southeastern Regional at Florida Atlantic University | Broward | |
| Pembroke Pines Charter High School | C | 7 | 7 | Southeastern Regional at Florida Atlantic University | Broward |
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| Pine Ridge High School | C | 1 | 1 | Eastern Regional at Stetson University | Volusia | |
| Pines Charter Central Middle | B | 2 | | Southern Regional at University of Miami | Broward | |
| Renaissance Charter School | B | 1 | | Southeastern Regional at Florida Atlantic University | Port St.Lucie | |
| River city Science Academy Innovation | B | 2 | 2 | Southeastern Regional at Florida Atlantic University | DUVAL |
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| River City Science Academy M-H | C | 3 | 3 | Eastern Regional at Stetson University | DUVAL |
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| River City Science Academy M-H | B | 3 | 3 | Eastern Regional at Stetson University | DUVAL |
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| River City Science Academy Mandarin | B | 1 | | Northwestern Regional at FSU: Panama City | DUVAL |
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| River City Science Academy Southeast | B | 2 | 2 | Western Regional at Hillsborough College, Brandon Campus | DUVAL |
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| River City Science Academy-Intracoastal | B | 3 | 3 | Eastern Regional at Stetson University | Duval | |
| River Springs Middle School | B | 2 | 1 | Eastern Regional at Stetson University | Volusia | |
| Sagemont Preparatory School | C | 1 | 1 | Southeastern Regional at Florida Atlantic University | Private | |
| Sarasota High School | C | 1 | 1 | Western Regional at Hillsborough College, Brandon Campus | Sarasota County School | |
| School for Advanced Studies- South | C | 2 | | Southern Regional at University of Miami | Miami-Dade County Public Schools | |
| Sebastian Charter Junior High | B | 1 | 1 | Southeastern Regional at Florida Atlantic University | Indian River | |
| Seminole High School | C | 2 | 2 | Eastern Regional at Stetson University | Seminole County Public Schools | |
| Seminole Science Charter School | B | 3 | 3 | Eastern Regional at Stetson University | Seminole |
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| Somerset Academy High School | C | 2 | | Southern Regional at University of Miami | Broward County Public Schools | |
| Somerset College Prep Academy | B | 1 | | Southeastern Regional at Florida Atlantic University | St. Lucie | |
| Somerset College Prep Academy | C | 1 | | Southeastern Regional at Florida Atlantic University | St. Lucie | |
| Soundside High School | C | 1 | | Central Regional at Osceola High School | Santa Rosa | |
| Spruce Creek High School | C | 3 | 3 | Eastern Regional at Stetson University | Volusia County School District |
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| St. Bonaventure Catholic School | B | 1 | | Southern Regional at University of Miami | Archdiocese of Miami | |
| Strawberry Crest High School | C | 3 | | Western Regional at Hillsborough College, Brandon Campus | Hillsborough County | |
| Suncoast Community High School | C | 2 | 2 | Southeastern Regional at Florida Atlantic University | Palm Beach School District | |
| Swift Creek Middle School | B | 1 | 1 | Northern Regional at The University of Florida | Leon County School District | |
| Taylor County Middle School | B | 1 | | Northwestern Regional at FSU: Panama City | Taylor County | |
| The Benjamin Upper School | C | 2 | | Southeastern Regional at Florida Atlantic University | Palm Beach | |
| The Villages Charter Middle School | B | 2 | 2 | Northern Regional at The University of Florida | Sumter | |
| The Villages High School | C | 1 | 1 | Central Regional at Osceola High School | Sumter | |
| Trinity Preparatory School of Florida (US) | C | 2 | 2 | Central Regional at Osceola High School | Independent | |
| University High School | C | 1 | 1 | Eastern Regional at Stetson University | Orange | |
| Velocity Raptors (Homeschool Team) | C | 1 | 1 | Southeastern Regional at Florida Atlantic University | Palm Beach County | |
| Velocity Raptors (Homeschool Team) | B | 1 | 1 | Southeastern Regional at Florida Atlantic University | Palm Beach County | |
| Viera High School | C | 1 | | Central Regional at Osceola High School | Brevard | |
| Walton Middle School | B | 2 | 2 | Northwestern Regional at FSU: Panama City | Walton | |
| Westminster Academy | C | 1 | 1 | Southern Regional at University of Miami | n/a | |
| Wiregrass Ranch High School | C | 2 | 2 | Western Regional at Hillsborough College, Brandon Campus | Pasco | |