| | | | | |
| Alexander W. Dreyfoos School of the Arts | C | 2 | Southeast Regional at Florida Atlantic University | School District of Palm Beach County | |
| All Saints Academy | B | 1 | Central Regional at Olympia High School (Orlando) | Independent | |
| Allen D Nease High School | C | 2 | Eastern Regional at Embry Riddle Aeronautical University | St Johns | |
| American Heritage | A | 1 | Southeast Regional at Florida Atlantic University | Palm Beach | |
| American Heritage Plantation | A | 1 | Southeast Regional at Florida Atlantic University | American Heritage | |
| American Heritage Plantation | C | 3 | Southeast Regional at Florida Atlantic University | Broward | |
| American Heritage Plantation | B | 2 | Southeast Regional at Florida Atlantic University | Broward | |
| American Heritage School | C | | Southeast Regional at Florida Atlantic University | Palm Beach | |
| American Heritage School Palm Beach | B | 1 | Southeast Regional at Florida Atlantic University | PBC/Private School | |
| Archbishop McCarthy High School | C | 2 | Southwest Regional at Canterbury School | Archdiocese of Miami | |
| Archimedean Academy | A | 1 | Southeast Regional at Florida Atlantic University | Miami-Dade | |
| Archimedean Middle Conservatory | B | 3 | Southeast Regional at Florida Atlantic University | Miami-Dade | |
| Archimedean Upper Conservatory | C | 4 | Southeast Regional at Florida Atlantic University | Miami-Dade | |
| Atlantic Coast High School | C | | Central Regional at Olympia High School (Orlando) | Duval County Public Schools | |
| Bartow International Baccalaureate | C | 1 | Western Regional at Hillsborough College, Brandon Campus | Polk | |
| Bartram Trail High School | C | 3 | Eastern Regional at Embry Riddle Aeronautical University | St. Johns | |
| Bay Meadows Elementary | A | 1 | ESO Central Regional | Orange County Public Schools | |
| Beachland Elementary School | A | 2 | Southeast Regional at Florida Atlantic University | Indian River County | |
| Boca Raton Community High School | C | 4 | Southeast Regional at Florida Atlantic University | Palm Beach | |
| Broward Math and Science Schools | B | | Southeast Regional at Florida Atlantic University | Broward | |
| Buchholz High School | B | 1 | Northern Regional at Buchholz High School, Gainesville | Alachua County Public Schools | |
| Buchholz High School | C | 3 | Northern Regional at Buchholz High School, Gainesville | Alachua County Public Schools | |
| Burns Scitech Charter School | B | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Caloosa Middle School | B | 1 | Southwest Regional at Canterbury School | School District of Lee County | |
| Canterbury School | C | 2 | Southwest Regional at Canterbury School | Private | |
| Carwise Middle School | B | 1 | Western Regional at Hillsborough College, Brandon Campus | Pinellas | |
| Celebration High School | C | 2 | Central Regional at Olympia High School (Orlando) | Osceola County Public Schools | |
| Celestina Home School | B | 1 | Eastern Regional at Embry Riddle Aeronautical University | Saint Johns | |
| Challenger Middle School | B | 1 | Southwest Regional at Canterbury School | School District of Lee County | |
| Chaminade-Madonna College Preparatory | C | 1 | Southeast Regional at Florida Atlantic University | Private | |
| Columbia City Elementary | A | | North FL Elementary in Lake City | Columbia County | |
| Columbia High School | C | 1 | Northern Regional at Buchholz High School, Gainesville | Columbia School District | |
| Community School of Naples | C | 2 | Southwest Regional at Canterbury School | private | |
| Community School of Naples | B | 1 | Southwest Regional at Canterbury School | Collier County | |
| Cooper City High School | C | | | Broward | |
| Coral Glades High School | C | 2 | Southeast Regional at Florida Atlantic University | Broward County Public Schools | |
| Country Day World School | B | 1 | Western Regional at Hillsborough College, Brandon Campus | Private | |
| Creekside High School | C | | | Saint Johns County School District |
|
| Cypress Creek High School | C | 1 | Central Regional at Olympia High School (Orlando) | Orange County Public Schools | |
| DELAND HIGH SCHOOL | C | 2 | Eastern Regional at Embry Riddle Aeronautical University | VOLUSIA | |
| Deltona High School | C | 2 | Eastern Regional at Embry Riddle Aeronautical University | Volusia County | |
| Deltona Middle School STEM Academy | B | 2 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Discovery Academy of Science | A | 2 | ESO Central Regional | Pinellas County Schools | |
| Discovery Academy of Science | B | 2 | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| Dommerich Elementary School | A | 1 | ESO Central Regional | Orange County Public Schools | |
| Dr Phillips High School | C | 1 | Central Regional at Olympia High School (Orlando) | Orange County | |
| Dunbar High School | C | 1 | Southwest Regional at Canterbury School | School District of Lee County | |
| Duncan U. Fletcher High School | C | 1 | Eastern Regional at Embry Riddle Aeronautical University | Duval | |
| Dunedin Highland Middle School | B | | | Pinellas County Schools | |
| East Lake High school | C | 1 | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| Everglades High School | C | 2 | Southern Regional at University of Miami | Broward | |
| Fairview Middle School | B | 3 | Northern Regional at Buchholz High School, Gainesville | Leon County |
|
| Florida Atlantic University High School | C | 3 | Southeast Regional at Florida Atlantic University | FAU Schools | |
| Florida State University Schools | C | 1 | Northern Regional at Buchholz High School, Gainesville | Florida State University Schools | |
| Fort Myers High School | C | 2 | Southwest Regional at Canterbury School | School District of Lee County | |
| Fort White Elementary School | A | | North FL Elementary in Lake City | Columbia County Schools | |
| Galaxy Middle School | B | 4 | Eastern Regional at Embry Riddle Aeronautical University | Volusia |
|
| Hagerty High School | C | 1 | Eastern Regional at Embry Riddle Aeronautical University | Seminole County | |
| Heritage Middle School | B | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia County School District | |
| Hialeah Gardens High School | C | 1 | Southern Regional at University of Miami | Miami-Dade County Public Schools | |
| Holy Trinity Episcopal Academy | C | 1 | Central Regional at Olympia High School (Orlando) | Private | |
| Home Education Boca Buddies | A | 1 | Southeast Regional at Florida Atlantic University | Palm Beach County | |
| Home Education Boca Buddies | B | 1 | Southeast Regional at Florida Atlantic University | Palm Beach County | |
| Horizon High School | C | 2 | Central Regional at Olympia High School (Orlando) | Orange | |
| Howard W. Blake High School | C | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough County Public Schools | |
| iBuild Academy, Inc | B | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| James S. Rickards High School | C | 6 | Northern Regional at Buchholz High School, Gainesville | Leon County | |
| Joe. E, Newsome high School | C | 2 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough county public Schools | |
| Lake Nona High School | C | 2 | Central Regional at Olympia High School (Orlando) | Orange | |
| Land O Lakes High School | C | 3 | Western Regional at Hillsborough College, Brandon Campus | Pasco County School District | |
| Largo High School | C | 2 | Western Regional at Hillsborough College, Brandon Campus | Pinellas County Schools | |
| Lawton Chiles High School | C | 2 | Northern Regional at Buchholz High School, Gainesville | Leon County | |
| Liberty High School | C | | Central Regional at Olympia High School (Orlando) | Osceola School District | |
| Maclay School | C | 1 | Northern Regional at Buchholz High School, Gainesville | Private | |
| Mainland High School | C | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Marjory Stoneman Douglas High School | C | 1 | Southeast Regional at Florida Atlantic University | Broward County | |
| Mary Help of Christians | B | 1 | Southern Regional at University of Miami | Archdiocese of Miami | |
| Melrose Park Elementary School | B | | Northern Regional at Buchholz High School, Gainesville | Columbia County | |
| Melrose Park ES | A | | North FL Elementary in Lake City | Columbia County | |
| Middleton High School | C | | Western Regional at Hillsborough College, Brandon Campus | Hillsborough county public schools | |
| Midtown Classical | B | | | Leon | |
| Moss Park Elementary | A | 1 | ESO Central Regional | Orange | |
| Narcoossee Middle | B | 1 | Central Regional at Olympia High School (Orlando) | Osceola | |
| Navarre High School - Florida | C | 4 | Eastern Regional at Embry Riddle Aeronautical University | Santa Rosa | |
| New Springs Schools | B | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough county schools | |
| Niblack Elementary School | A | | North FL Elementary in Lake City | Columbia County | |
| North Elementary | A | 1 | ESO Central Regional | Okeechobee County | |
| North Fort Myers High School | C | 1 | Southwest Regional at Canterbury School | Lee County School District | |
| Nova HIgh | C | | | School Board of Broward County | |
| Oak Hall School | C | 1 | Northern Regional at Buchholz High School, Gainesville | Oak Hall School | |
| Odyssey Charter School | B | | Central Regional at Olympia High School (Orlando) | Brevard Public Schools | |
| OLSEN MIDDLE SCHOOL | B | 2 | Southern Regional at University of Miami | BROWARD COUNTY PUBLIC SCHOOLS | |
| Olympia High School | C | 7 | Central Regional at Olympia High School (Orlando) | Orange County Public Schools | |
| Olympic Heights High School | C | 1 | Southeast Regional at Florida Atlantic University | Palm Beach School District | |
| Orlando Gifted Academy | A | | ESO Central Regional | Orange County Public Schools | |
| Orlando Gifted Academy | B | 1 | Central Regional at Olympia High School (Orlando) | Orange County Public Schools | |
| Orlando Science Elementary School | A | 6 | First Coast Elementary Regional at River City Science Academy | Orange County | |
| Orlando Science High School | C | 6 | Central Regional at Olympia High School (Orlando) | Orange | |
| Orlando Science Middle School | B | 7 | Central Regional at Olympia High School (Orlando) | Orange |
|
| Osceola High School | C | 4 | Central Regional at Olympia High School (Orlando) | School District of Osceola County | |
| Osceola Science Charter School | A | 2 | Southeast Regional at Florida Atlantic University | Osceola | |
| Osceola Science Charter School | B | 2 | Eastern Regional at Embry Riddle Aeronautical University | Osceola District | |
| P. K. Yonge Developmental Research School | C | 1 | Northern Regional at Buchholz High School, Gainesville | University of Florida Lab School | |
| Palm Beach Central High School | C | 1 | Southeast Regional at Florida Atlantic University | Palm Beach | |
| Palm Beach Day Academy | B | | Southeast Regional at Florida Atlantic University | Private | |
| Palm Harbor | C | 2 | Western Regional at Hillsborough College, Brandon Campus | Largo | |
| Pembroke Pines Charter Academic Village | B | 2 | Southeast Regional at Florida Atlantic University | Broward | |
| Pembroke Pines Charter Central Middle School | B | 5 | Southeast Regional at Florida Atlantic University | Broward | |
| Pembroke Pines Charter High School | C | 4 | Southeast Regional at Florida Atlantic University | Broward | |
| Pensacola High School | C | 1 | Northern Regional at Buchholz High School, Gainesville | Escambia |
|
| Pine Ridge High School | C | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Pine View School | C | 1 | Western Regional at Hillsborough College, Brandon Campus | Sarasota | |
| Pine View School | B | 2 | Western Regional at Hillsborough College, Brandon Campus | Sarasota | |
| Randall Middle School | B | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough County Public Schools | |
| Rise Academy | B | | Southeast Regional at Florida Atlantic University | Broward | |
| Rise Academy School | A | 1 | Southeast Regional at Florida Atlantic University | Rise Academy School of Science and Technology | |
| RISE Academy School of Science and Technology | B | 1 | Southeast Regional at Florida Atlantic University | Broward | |
| River City Science Academy at Intracoastal | A | 1 | North FL Elementary in Lake City | Duval | |
| River City Science Academy Elementary | A | 2 | North FL Elementary in Lake City | Duval | |
| River city Science Academy Innovation | B | 1 | Eastern Regional at Embry Riddle Aeronautical University | DUVAL | |
| River City Science Academy Innovation | A | 1 | First Coast Elementary Regional at River City Science Academy | Duval | |
| River City Science Academy Intracoastal | A | 2 | First Coast Elementary Regional at River City Science Academy | Duval | |
| River City Science Academy Intracoastal | B | | | River City Science Academy | |
| River City Science Academy M-H | B | 4 | Eastern Regional at Embry Riddle Aeronautical University | DUVAL |
|
| River City Science Academy M-H | C | 2 | Eastern Regional at Embry Riddle Aeronautical University | DUVAL |
|
| River City Science Academy Mandarin | B | 1 | Northern Regional at Buchholz High School, Gainesville | DUVAL |
|
| River City Science Academy Mandarin | A | 2 | North FL Elementary in Lake City | DUVAL | |
| River City Science Academy-Intracoastal | B | 1 | Eastern Regional at Embry Riddle Aeronautical University | Duval | |
| River Springs Middle School | B | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Roberto Clemente Middle School | B | 2 | Central Regional at Olympia High School (Orlando) | Orange County | |
| Saint Petersburg High School | C | | | Pinellas County Schools | |
| Sanders Park Elementary | A | 1 | Southeast Regional at Florida Atlantic University | Broward County | |
| School for Advanced Studies- South | C | 1 | Southern Regional at University of Miami | Miami-Dade County Public Schools | |
| School for Advanced Studies- West | C | 1 | Southern Regional at University of Miami | Miami-Dade County Public Schools | |
| Seabreeze High School | C | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia County Schools | |
| Seminole Elementary School (Okeechobee, FL School District) | A | 1 | ESO Central Regional | Okeechobee | |
| Seminole High School | C | 1 | Central Regional at Olympia High School (Orlando) | Seminole County Public Schools | |
| Seminole Science | A | 2 | ESO Central Regional | Seminole | |
| Seminole Science Charter School | B | 2 | Eastern Regional at Embry Riddle Aeronautical University | Seminole |
|
| Seminole Science Charter School | A | 2 | First Coast Elementary Regional at River City Science Academy | Seminole County Public Schools | |
| silver Sands Middle School | B | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia | |
| Somerset Academy Boca East | A | 1 | Southeast Regional at Florida Atlantic University | Palm Beach | |
| Somerset Academy High School | C | 1 | Southern Regional at University of Miami | Broward | |
| Somerset College Prep Academy | C | 1 | Eastern Regional at Embry Riddle Aeronautical University | St. Lucie | |
| South Broward High School | C | 1 | Southern Regional at University of Miami | Broward Schools | |
| South Elementary School | A | 1 | Southeast Regional at Florida Atlantic University | Okeechobee County Schools | |
| Spanish River High School | C | 2 | Southeast Regional at Florida Atlantic University | Palm Beach County School District | |
| Spruce Creek High School | C | 3 | Eastern Regional at Embry Riddle Aeronautical University | Volusia County School District |
|
| St. Bonaventure Catholic School | A | 1 | Southeast Regional at Florida Atlantic University | Archdiocese of Miami | |
| St. Bonaventure Catholic School | B | 1 | Southern Regional at University of Miami | Archdiocese of Miami | |
| St. Johns Country Day School | C | 1 | Eastern Regional at Embry Riddle Aeronautical University | Private | |
| St. Johns Country Day School | B | 1 | Eastern Regional at Embry Riddle Aeronautical University | Private | |
| St. Thomas Aquinas High School | C | 2 | Southern Regional at University of Miami | Archdiocese of Miami | |
| St.Petersburg High School | C | 1 | Western Regional at Hillsborough College, Brandon Campus | Pinellas County | |
| Stanton College Preparatory | C | 2 | Northern Regional at Buchholz High School, Gainesville | Duval County Public Schools | |
| Steinbrenner High School | C | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough County Public School | |
| Strawberry Crest High School | C | 3 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough County | |
| Strawberry Crest High School | C | | | Hillsborough | |
| SUMMERS ELEMENTARY | A | | North FL Elementary in Lake City | COLUMBIA | |
| Suncoast High School | C | 2 | Southeast Regional at Florida Atlantic University | Palm Beach County | |
| Swift Creek Middle School | B | 1 | Northern Regional at Buchholz High School, Gainesville | Leon | |
| Tallahasse School of Math and Science | B | 1 | Northern Regional at Buchholz High School, Gainesville | Leon County | |
| Tampa Homeschoolers Instructing and Nurturing Kids | A | 1 | North FL Elementary in Lake City | Homeschool | |
| Tampa Preparatory School | C | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough | |
| TEST-McKee | C | | Eastern Regional at Embry Riddle Aeronautical University | TEST | |
| The Discovery School | B | 1 | Eastern Regional at Embry Riddle Aeronautical University | Duval | |
| Timber Creek High School | C | 2 | Central Regional at Olympia High School (Orlando) | Orange County Public Schools | |
| Trinity Preparatory School | C | 2 | Central Regional at Olympia High School (Orlando) | Independent School | |
| Universal Academy of Florida | B | 1 | Western Regional at Hillsborough College, Brandon Campus | Hillsborough | |
| University High School | C | 2 | Eastern Regional at Embry Riddle Aeronautical University | Orange | |
| University High School | C | 1 | Eastern Regional at Embry Riddle Aeronautical University | Volusia County Schools | |
| Villages High School | C | 1 | Central Regional at Olympia High School (Orlando) | Sumter | |
| West Broward High School | C | 4 | Southeast Regional at Florida Atlantic University | Broward County | |
| Westside Elementary School | A | | North FL Elementary in Lake City | Columbia County | |
| Windermere Elementary School | A | 1 | ESO Central Regional | Orange County Public Schools | |
| Windermere High School | C | 1 | Central Regional at Olympia High School (Orlando) | Orange county | |
| Winter Park High School | C | 1 | Central Regional at Olympia High School (Orlando) | Orange County Public Schools | |