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| Allendale Public Middle School | B | 1 | 1 | Division 12 | Allendale Public Schools | MI | |
| Arcadia Public Schools | B | 2 | 2 | ESU 10 Kearney | Arcadia Public Schools | NE |
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| BASIS Cedar Park | B | 1 | 1 | Texas UT Austin | Charter | TX |
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| Chaboya MS | B | 1 | 1 | Santa Clara County Regional Science Olympiad | Evergreen School District | CA |
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| Chapin School (MS) | B | 2 | 2 | New Jersey Science Olympiad | Private | NJ | |
| Discovery Peak Charter School | B | 1 | 1 | Alaska Science Olympiad | Fairbanks North Star Borough School District | AK | |
| Georgina P Blach Intermediate School | B | 2 | 2 | Santa Clara County Regional Science Olympiad | Los Altos School District | CA | |
| Noe Middle School | B | 2 | 2 | Louisville Regional | Jefferson County Schools | KY | |
| Odle Middle School | B | 1 | 1 | Regional - NW-B-East | Bellevue School District | WA | |
| Quail Valley Middle School | B | 2 | 2 | Gulf Coast | Fort Bend ISD | TX | |
| River Trail Middle School | B | 3 | 3 | Regional Placeholder | Fulton County | GA |
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| Shaker Middle School | B | 2 | 2 | | North colonie school district | NY | |
| St. Mary Catholic | B | 2 | 2 | Wilmington | Diocese of Raleigh | NC |
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| Stoller Middle School | B | 3 | 3 | Oregon Science Olympiad | Beaverton School District | OR |
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| Timberline Middle School | B | 1 | 1 | NW Div B East | Lake Washington School District | WA |
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| Travis Middle School | B | 1 | 1 | Travis Middle School | McAllen ISD | TX | |
| Tuckahoe Middle School | B | 3 | 3 | | Tuckahoe Union Free School District | NY | |
| Tumwater Cosmic Catalysts | B | 1 | 1 | Tumwater Middle School | BSD | OR | |
| Turtle Rock Elementary School | B | 2 | 2 | Orange County | Irvine Unified School District | CA | |
| Urbana Middle School | B | 1 | 1 | | Frederick County Public Schools | MD | |
| Venado Middle School | B | 1 | 1 | Southern California | Irvine Unified School District | CA |
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| Westpark Elementary School | B | 3 | 3 | Orange County | Irvine Unified School District | CA | |