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| Altamont ES | B | 2 | | Lammersville Unified School District | CA | |
| BASIS Austin | B | 2 | | BASIS Texas | TX | |
| Fairmont Private Schools - Anaheim Hills | B | 3 | SoCal Joint Regional | Private | CA |
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| Hawthorn Middle South | B | 2 | Illinois Regional | Hawthorn District 73 | IL | |
| Jeffrey Trail Middle School | B | 3 | SoCal Joint Regional | Irvine Unified School District | CA | |
| Kealing Middle School | B | 2 | UT Austin | Austin ISD | TX | |
| Kennedy MS | B | 9 | | Cupertino Union School District | CA |
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| Longfellow Middle School | B | 2 | | Fairfax County Public Schools | VA |
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| Marshall Middle School | B | 4 | SDSO | San Diego Unified School District | CA | |
| Orange County School of the Arts | B | 3 | Orange County | Independent Charter | CA | |
| Prattville Christian Academy Jr. High School | B | 2 | AMSTI-North Alabama Regional | Private | AL |
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| Redmond Middle School | B | 4 | Regional - Northwest (Division B) | Lake Washington School District | WA | |
| Redwood MS | B | | Santa Clara County Regional Science Olympiad | Saratoga Union School District | CA | |
| Sierra Vista Middle School | B | 12 | | IUSD | CA |
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| Stoller Middle School | B | 1 | Oregon Science Olympiad | Beaverton School District | OR |
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| Timberline Middle School | B | 5 | Regional - Southwest (Division B) | Lake Washington School District | WA |
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