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| Benton Central High School | C | 1 | Ivy Tech Lafayette Regional | IN | |
| Bloomington High School North | C | 3 | Ivy Tech Lafayette Regional | IN |
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| Bloomington High School South | C | 2 | Butler University Regional | IN |
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| Carmel High School | C | 3 | Butler University Regional | IN |
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| Centerville High School | C | 3 | Western Ohio Regional Tournament | OH | |
| Clay Middle School | B | 2 | Butler University Regional | IN | |
| College Preparatory School of America | C | 2 | College of Dupage | IL | |
| Columbia City High School | C | 1 | Manchester University | IN | |
| Hadley E. Watts Middle School | B | 3 | Western Ohio Regional Tournament | OH | |
| Hamilton Southeastern High School | C | 2 | Butler University Regional | IN |
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| Harshman Middle School | B | 2 | Manchester University | IN | |
| Hawthorn Middle North | B | 1 | Illinois Regional | IL | |
| Jackson Creek Middle School | B | 3 | Butler University Regional | IN | |
| John Adams H. S. | C | 2 | Valpo Regional | IN | |
| Lake Central High School | C | 2 | Valpo Regional | IN | |
| Magsig Middle School | B | 3 | Western Ohio Regional Tournament | OH |
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| Mason High School | C | 3 | Southwestern Ohio Science Olympiad | OH |
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| Mason Intermediate | B | 3 | Southwestern Ohio Science Olympiad | OH | |
| Mason Middle School | B | 3 | Southwestern Ohio Science Olympiad | OH |
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| Munster HS | C | 2 | Purdue University Northwest Regional | IN | |
| North Central High School | C | 2 | Ivy Tech Lafayette Regional | IN | |
| Northmont High School | C | 1 | Western Ohio Regional Tournament | OH | |
| Northmont middle school | B | 2 | Western Ohio Regional Tournament | OH | |
| Patterson Park Homeschoolers | C | 1 | | OH | |
| Raymond Park Intermediate and Middle School | B | 2 | Butler University Regional | IN | |
| Science & Arts Academy | B | 1 | Oakton (TBD) | IL |
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| St. Simon The Apostle | B | 1 | Butler University Regional | IN | |
| Tower Heights Middle School | B | 1 | Western Ohio Regional Tournament | OH |
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| Twin Valley South High School | C | 1 | Western Ohio Regional Tournament | OH | |
| Twin Valley South Middle School | B | 2 | Western Ohio Regional Tournament | OH | |
| Warren Central High School | C | 1 | Butler University Regional | IN | |
| Wilbur Wright Middle School | B | 2 | Purdue University Northwest Regional | IN |
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| Wyoming Middle School | B | 1 | Southwestern Ohio Science Olympiad | OH | |