| | | | | |
| Albany Middle School | B | 8 | | Alameda Regional Science Olympiad | CA |
| Alston Ridge Middle School | B | 1 | 1 | Raleigh Regional | NC |
| Archimedean Middle Conservatory | B | 3 | 3 | Southeast Regional at Florida Atlantic University | FL |
| BASIS Cedar Park | B | 8 | | Texas UT Austin | TX |
| BASIS Independent Fremont (MS) | B | 3 | 3 | Alameda Regional Science Olympiad | CA |
| Basis Pflugerville | B | 4 | 4 | n/a | TX |
| Beckendorff Junior High | B | 2 | 2 | University of Texas Regional | TX |
| Carden Park Elementary School | B | 1 | | Region 3 B/C | MO |
| Cedarlane Academy | B | 2 | | SoCal | CA |
| Dana Middle School | B | 3 | 3 | SoCal Joint Regional | CA |
| Galaxy Middle School | B | 3 | | Eastern Regional at Stetson University | FL |
| Garnet Valley MS | B | 3 | 3 | Southeast Regional at PSU Abington | PA |
| Henry E. Huntington Middle School | B | 3 | 3 | CA | CA |
| Hesston Middle School | B | 2 | | Wichita Regional at Andover Division B | KS |
| Jeffrey Trail Middle School | B | 4 | | Orange County Regional | CA |
| Julia Landon College Prepatory and Leadership Development Middle School | B | 1 | | Northern Regional at The University of Florida | FL |
| Lake Highland Preparatory School | B | 1 | 1 | Central Regional at Osceola High School | FL |
| North Pocono Middle School | B | 3 | | Northeast Region at PSU Wilkes-Barre | PA |
| Rancho San Joaquin Middle School | B | 2 | | Orange | CA |
| Redwood Middle School | B | 3 | | | CA |
| River city Science Academy Innovation | B | 2 | | Southeast Regional at Florida Atlantic University | FL |
| River City Science Academy Southeast | B | 2 | 2 | Western Regional at Hillsborough College, Brandon Campus | FL |
| River City Science Academy-Intracoastal | B | 3 | | Eastern Regional at Stetson University | FL |
| Sierra Vista Middle School | B | 5 | | Orange County | CA |
| Steam Academy | B | 1 | 1 | San Joaquin County | CA |
| The Pingry School | B | 3 | 3 | | NJ |
| The Shipley School B | B | 2 | 2 | Southeast Regional at PSU Abington | PA |