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| Anna Middle School | B | 2 | | Western Ohio Regional Tournament | Anna Local Schools | OH | |
| Arbor Hills Junior High | B | 1 | | BGSU Northwest Ohio Regional | Sylvania Schools | OH | |
| Canal Winchester Middle School | B | 2 | | Central Ohio Regional Tournament | Canal Winchester Local Schools | OH | |
| Daniel Wright Junior High School | B | 2 | | Illinois Regional | Lincolnshire-Prairie View District 103 | IL | |
| Galaxy Middle School | B | 1 | | Div B: Southern | Geone | CA | |
| Grandview Heights Larson MS | B | 2 | | Central Ohio Regional Tournament | Grandview Heights | OH | |
| Granville Middle School | B | 2 | | Central Ohio Regional Tournament | Granville Exempted Village School District | OH | |
| Hadley E Watts Middle School | B | 3 | | Western Ohio Regional Tournament | Centerville City Schools | OH |
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| Hudson Middle School | B | 3 | | Northern Ohio Regional | Hudson City Schools | OH |
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| Kenston Middle School | B | 1 | | Northeast & Northern Ohio Regional Tournament | Kenston Local Schools | OH |
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| Magsig Middle School | B | 3 | | Western Ohio Regional Tournament | Centerville City Schools | OH |
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| Mars Hill Academy Blue | B | 1 | | Southwestern | Mason City Schools | OH |
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| Mason Intermediate | B | 3 | | Southwestern Ohio Science Olympiad | Mason City Schools | OH | |
| Mason Middle School | B | 2 | | Southwestern Ohio Science Olympiad | Mason City Schools | OH |
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| McCord JH | B | 1 | | BGSU Northwest Ohio Regional | Sylvania Schools | OH | |
| New Albany Middle School | B | 2 | | Central Ohio Regional Tournament | New Albany Plain Local School District | OH | |
| Northmont middle school | B | 2 | | Western Ohio Regional Tournament | Northmont city schools | OH | |
| Olentangy Orange Middle School | B | 2 | | Central Ohio Regional Tournament | Olentangy Local Schools | OH | |
| Piqua Junior High School | B | 1 | | Western Ohio Regional Tournament | Piqua City Schools | OH | |
| Raymond Park Intermediate and Middle School | B | 2 | | State | MSD Warren Township | IN | |
| Solon Middle School | B | 2 | | Northern Ohio Regional | Solon City Schools | OH | |
| St. Charles Borromeo | B | 2 | | Western Ohio Regional Tournament | Archdiocese of Cincinnati | OH | |
| Twin Valley South Middle School | B | 1 | | Western Ohio Regional Tournament | Twin Valley Community Local | OH | |
| West Liberty-Salem Middle School | B | 2 | | Western Ohio Regional Tournament | West Liberty-Salem Schools | OH | |
| Wyoming Middle School | B | 2 | | Southwestern Ohio Science Olympiad | Wyoming City Schools | OH | |